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Cell sorting is the process through which a particular cell type is separated from others contained in a sample on the basis of its physical or biological properties, such as size, morphological parameters, viability and both extracellular and intracellular protein expression.
Magnetic-activated cell sorting is a commonly used method in areas like immunology, cancer research, neuroscience, and stem cell research. Miltenyi sells microbeads which are magnetic nanoparticles conjugated to antibodies which can be used to target specific cells.
Cells are labeled with primary antibodies and then MACS beads are coated with specific- specific antibodies. These labeled cell suspension is then put into a separation column in a strong magnetic field. The labeled cells are contained, magnetized, while in the magnetic field and the unlabeled cells are suspended, un-magnetized, to be collected.
Protein targeting or protein sorting is the biological mechanism by which proteins are transported to their appropriate destinations within or outside the cell. [1] [2] [note 1] Proteins can be targeted to the inner space of an organelle, different intracellular membranes, the plasma membrane, or to the exterior of the cell via secretion.
Fluorescence-activated cell sorting (FACS) is a technique for sorting out the cells based on the differences that can be detected by light scatter (e.g. cell size) or fluorescence emission (by penetrated DNA, RNA, proteins or antigens). The system works much like flow cytometry, but will also charge each cell droplet after it has been measured ...
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With Confluent's data streaming platform, the telecom provider streams mobile data from 70,000 U.S. cell towers with connectors feeding data into lakes and warehouses.