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Ascorbic acid is easily oxidized and so is used as a reductant in photographic developer solutions (among others) and as a preservative. [citation needed] In fluorescence microscopy and related fluorescence-based techniques, ascorbic acid can be used as an antioxidant to increase fluorescent signal and chemically retard dye photobleaching. [29]
The Reichstein process in chemistry is a combined chemical and microbial method for the production of ascorbic acid from D-glucose that takes place in several steps. [1] This process was devised by Nobel Prize winner Tadeusz Reichstein and his colleagues in 1933 while working in the laboratory of the ETH in Zürich.
Another formula consists of indigo carmine, ascorbic acid , sodium bicarbonate, sodium chloride, copper(II) sulfate, sodium hydroxide and water. [17] By doing so, chemical waste and the level of corrosive chemicals is reduced. The amount of solid chemicals dissolved in the experiment could be reduced from 60 grams to 6 grams.
Ascorbic acid efflux by embryos of dicot plants is a well-established mechanism of iron reduction and a step obligatory for iron uptake. [a] All plants synthesize ascorbic acid. Ascorbic acid functions as a cofactor for enzymes involved in photosynthesis, synthesis of plant hormones, as an antioxidant and regenerator of other antioxidants. [96]
Dehydroascorbic acid (DHA) is an oxidized form of ascorbic acid (vitamin C). It is actively imported into the endoplasmic reticulum of cells via glucose transporters. [ 1 ] It is trapped therein by reduction back to ascorbic acid by glutathione and other thiols . [ 2 ]
[1] [2] If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid. This reaction is a redox reaction: vitamin C (ascorbic acid) is oxidized to dehydroascorbic acid, and DCPIP is reduced to the colorless compound DCPIPH 2
The G6PD deficiency test is a common laboratory test. Because oxalic acid is produced during metabolism of vitamin C, hyperoxaluria can be caused by intravenous administration of ascorbic acid. [20] Vitamin C administration may also acidify the urine and could promote the precipitation of kidney stones or drugs in the urine. [20]
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