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Dishcloths are often left damp and provide a breeding ground for bacteria. Since the kitchen sink is used to clean food, dishcloths are routinely infected with E. coli and salmonella. In 2007, a study from the Journal of Environmental Health found that putting a damp dishcloth (or sponge) in the microwave for 2 minutes killed 99% of living ...
An important factor contributing to the stability of the construct in step I is a high GC-content at the 3' ends and length of the overlap. The third step occurs in the next cycle, when a single strand of the product of step II is used as a template to which fresh primers anneal leading to synthesis of more PD product. [1]
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