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The reflectance of the surface of a material is its effectiveness in reflecting radiant energy. It is the fraction of incident electromagnetic power that is reflected at the boundary. It is the fraction of incident electromagnetic power that is reflected at the boundary.
Diffuse reflectance spectroscopy, or diffuse reflection spectroscopy, is a subset of absorption spectroscopy.It is sometimes called remission spectroscopy.Remission is the reflection or back-scattering of light by a material, while transmission is the passage of light through a material.
The transmittance or reflectance value for each wavelength of the test sample is then compared with the transmission or reflectance values from the reference sample. Most instruments will apply a logarithmic function to the linear transmittance ratio to calculate the 'absorbency' of the sample, a value which is proportional to the ...
Reflectance difference spectroscopy (RDS) is a spectroscopic technique which measures the difference in reflectance of two beams of light that are shone in normal incident on a surface with different linear polarizations. [1] It is also known as reflectance anisotropy spectroscopy (RAS). [2] It is calculated as:
Diffuse reflectance spectroscopy can be used to determine the absorption spectra of powdered samples in cases where transmission spectroscopy is not feasible. This applies to UV-Vis-NIR spectroscopy or mid-infrared spectroscopy .
Infrared (IR) spectroscopy by ATR is applicable to the same chemical or biological systems as the transmission method. One advantage of ATR-IR over transmission-IR is the limited path length into the sample. This avoids the problem of strong attenuation of the IR signal in highly absorbing media such as aqueous solutions.
The spectra are plotted in units of log inverse reflectance (log 1/R) versus wavenumber. Alternative plots of Kubelka-Munk units can be used, which relate reflectance to concentration using a scaling factor. A reflectance standard is needed in order to quantify the reflectance of the sample because it cannot be determined directly. [2] [3]
Spectroscopy, primarily in the electromagnetic spectrum, is a fundamental exploratory tool in the fields of astronomy, chemistry, materials science, and physics, allowing the composition, physical structure and electronic structure of matter to be investigated at the atomic, molecular and macro scale, and over astronomical distances.