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Molecular breeding is the application of molecular biology tools, often in plant breeding [1] [2] and animal breeding. [3] [4] In the broad sense, molecular breeding can be defined as the use of genetic manipulation performed at the level of DNA to improve traits of interest in plants and animals, and it may also include genetic engineering or gene manipulation, molecular marker-assisted ...
[3] [20] For example, DNA shuffling has been used to increase the potency of phage-displayed recombinant interferons on murine and human cells. [3] Additionally, the improvement of green fluorescent protein (GFP) was accomplished with DNA shuffling by molecular breeding as a 45-fold greater signal than the standard for whole cell fluorescence ...
[1] [2] The first paper describing this software was published in 1989. [3] The program is a real time PCR primer and probe search and analysis tool. It additionally performs siRNA and molecular beacon searches, open reading frame analysis, and restriction enzyme analysis. It was created and maintained by Wojciech Rychlik and Piotr Rychlik.
About 10,000 years ago is when humans began to domesticate plant species for the purpose of food, seeds, and vegetation. [ 4 ] Since then, agriculture has been a staple for human civilizations and plant breeding has allowed more genetic diversity and a more diverse gene pool. [ 4 ]
Marker assisted selection or marker aided selection (MAS) is an indirect selection process where a trait of interest is selected based on a marker (morphological, biochemical or DNA/RNA variation) linked to a trait of interest (e.g. productivity, disease resistance, abiotic stress tolerance, and quality), rather than on the trait itself.
Plant breeders use different methods depending on the mode of reproduction of crops, which include: Self-fertilization, where pollen from a plant will fertilise reproductive cells or ovules of the same plant; Cross-pollination, where pollen from one plant can only fertilize a different plant
Other molecular breeding tool include development of genetically modified crops that can tolerate plant stress. As a complement to the recent rapid progress in genomics , a better understanding of physiological mechanisms of dehydration response will also contribute to the progress of genetic enhancement of crop drought resistance.
TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene.TILLING was introduced in 2000, using the model plant Arabidopsis thaliana, and expanded on into other uses and methodologies by a small group of scientists including Luca Comai.