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  2. Molecular diagnostics - Wikipedia

    en.wikipedia.org/wiki/Molecular_diagnostics

    PCR is currently the most widely used method for detection of DNA sequences. [22] The detection of the marker might use real time PCR, direct sequencing, [2]: ch 17 microarray chips—prefabricated chips that test many markers at once, [2]: ch 24 or MALDI-TOF [23] The same principle applies to the proteome and the genome.

  3. Multiplex ligation-dependent probe amplification - Wikipedia

    en.wikipedia.org/wiki/Multiplex_ligation...

    Ligase is applied to the hybridized DNA, combining probe pairs that are hybridized immediately next to each other into a single strand of DNA that can be amplified by PCR. PCR amplifies all probe pairs that have been successfully ligated, using fluorescently labeled PCR primers. The PCR products are quantified, typically by (capillary ...

  4. Cancer biomarker - Wikipedia

    en.wikipedia.org/wiki/Cancer_biomarker

    A biomarker may be used to see how well the body responds to a treatment for a disease or condition. Also called molecular marker and signature molecule." [18] In cancer research and medicine, biomarkers are used in three primary ways: [19] To help diagnose conditions, as in the case of identifying early stage cancers (diagnostic)

  5. Digital polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Digital_polymerase_chain...

    Chip-based Digital PCR (dPCR) is also a method of dPCR in which the reaction mix (also when used in qPCR) is divided into ~10,000 to ~45,000 partitions on a chip, then amplified using an endpoint PCR thermocycling machine, and is read using a high-powered camera reader with fluorescence filter (HEX, FAM, Cy5, Cy5.5 and Texas Red) for all ...

  6. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  7. Kompetitive allele specific PCR - Wikipedia

    en.wikipedia.org/.../Kompetitive_allele_specific_PCR

    Kompetitive allele specific PCR (KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific oligo extension and fluorescence resonance energy transfer for signal generation.

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