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The Kjeldahl method or Kjeldahl digestion (Danish pronunciation: [ˈkʰelˌtɛˀl]) in analytical chemistry is a method for the quantitative determination of a sample's organic nitrogen plus ammonia/ammonium (NH 3 /NH 4 +). Without modification, other forms of inorganic nitrogen, for instance nitrate, are not included in
His laboratory technique for nitrogen and protein analysis is still the universally accepted method for this analysis. Although other methods claim to be faster and more efficient, none can cope with the variety of sizes or conditions of samples than Johan Kjeldahl's original method. Kjeldahl equipment is used extensively all over the world.
The AutoAnalyzer is an automated analyzer using a flow technique called continuous flow analysis (CFA), or more correctly segmented flow analysis (SFA) first made by the Technicon Corporation. The instrument was invented in 1957 by Leonard Skeggs, PhD and commercialized by Jack Whitehead's Technicon Corporation.
The measured signal from the thermal conductivity detector for the unknown sample can then be converted into a nitrogen content. As with the Kjeldahl method, conversion of the concentration of nitrogen in a sample to the crude protein content is performed using conversion factors which depend on the particular amino acid sequence of the ...
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The sodium fusion test, or Lassaigne's test, is used in elemental analysis for the qualitative determination of the presence of foreign elements, namely halogens, nitrogen, and sulfur, in an organic compound. It was developed by J. L. Lassaigne. [1] The test involves heating the sample with sodium metal, "fusing" it with the sample. A variety ...
However, on food labels the protein is calculated by the nitrogen multiplied by 6.25, because the average nitrogen content of proteins is about 16%. The Kjeldahl test is typically used because it is the method the AOAC International has adopted and is therefore used by many food standards agencies around the world, though the Dumas method is ...
The Schlenk line (also vacuum gas manifold) is a commonly used chemistry apparatus developed by Wilhelm Schlenk. [1] It consists of a dual manifold with several ports. [ 2 ] One manifold is connected to a source of purified inert gas , while the other is connected to a vacuum pump .