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When the acoustic wave propagates though the sample it may be scattered, absorbed or reflected at media interfaces. Thus, the technique registers the echo generated by the acoustic impedance (Z) contrast between two materials. Scanning acoustic microscopy works by directing focused sound from a transducer at a small point on a target object.
The notion of acoustic microscopy dates back to 1936 when S. Ya. Sokolov [1] proposed a device for producing magnified views of structure with 3-GHz sound waves. However, due to technological limitations at the time, no such instrument could be constructed, and it was not until 1959 that Dunn and Fry [2] performed the first acoustic microscopy experiments, though not at very high frequencies.
This concept can be more conceivable by imagining it in analogy to laser theory. Theodore Maiman operated the first functioning LASER on May 16, 1960 at Hughes Research Laboratories, Malibu, California, [6] A device that operates according to the central idea of the "sound amplification by stimulated emission of radiation" theory is the thermoacoustic laser.
Photoacoustic imaging or optoacoustic imaging is a biomedical imaging modality based on the photoacoustic effect.Non-ionizing laser pulses are delivered into biological tissues and part of the energy will be absorbed and converted into heat, leading to transient thermoelastic expansion and thus wideband (i.e. MHz) ultrasonic emission.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Scanning near-field ultrasound holography combines atomic force acoustic microscopy and ultrasonic force microscopy. Two transducers producing high frequencies are used. Usually frequency is higher than the resonant frequency of the cantilever. One transducer is placed below the sample and the other attached to the cantilever.
AFAM. Atomic force acoustic microscopy (AFAM) is a type of scanning probe microscopy (SPM). It is a combination of acoustics and atomic force microscopy. The principal difference between AFAM and other forms of SPM is the addition of a transducer at the bottom of the sample which induces longitudinal out-of-plane vibrations in the specimen.
Edge filters are used for removal of all primary laser light. Resolution as low as 10 nm can be achieved using this technique. [citation needed] Near field infrared spectrometry and near-field dielectric microscopy [19] use near-field probes to combine sub-micron microscopy with localized IR spectroscopy. [25]