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A diaphonized mirror dory.The bones are dyed red and the cartilage is dyed blue. Diaphonized veiled chameleon. Field Museum of Natural History, Chicago.. Diaphonization (or diaphonisation), also known as clearing and staining, is a staining technique used on animal specimens that first renders the body of the animal transparent by bathing it in trypsin, and then stains the bones and cartilage ...
[1]) is a water-soluble sodium salt of Alizarin sulfonic acid with a chemical formula of C 14 H 7 NaO 7 S. [2] [1] Alizarin Red S was discovered by Graebe and Liebermann in 1871. [2] In the field of histology alizarin Red S is used to stain calcium deposits in tissues, [3] [4] and in geology to stain and differentiate carbonate minerals. [3]
Free (ionic) calcium forms precipitates with alizarin, and tissue block containing calcium stain red immediately when immersed in alizarin. Thus, both pure calcium and calcium in bones and other tissues can be stained. These alizarin-stained elements can be better visualized under fluorescent lights, excited by 440–460 nm. [18]
The number of pet dogs jumped as much as 16% between 2016 and 2020, while pet cats climbed as much as 6%, according to the American Veterinary Medical Association.
A dog exiting through a pet door. A pet door or pet flap (also referred to in more specific terms, such as cat flap, cat door, kitty door, dog flap, dog door, or doggy/doggie door) is a small opening to allow pets to enter and exit a building on their own without needing a human to open the door. Originally simple holes, the modern form is a ...
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