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The canonical example of attenuation used in many introductory genetics textbooks, [1] is ribosome-mediated attenuation of the trp operon. Ribosome-mediated attenuation of the trp operon relies on the fact that, in bacteria, transcription and translation proceed simultaneously. Attenuation involves a provisional stop signal (attenuator ...
A large part of gene regulation occurs through transcription factors that either recruit or inhibit the binding of the general transcription machinery and/or the polymerase. This can be accomplished through close interactions with core promoter elements, or through the long distance enhancer elements.
Transcription attenuation is a type of prokaryotic regulation that happens only under certain conditions. This process occurs at the beginning of RNA transcription and causes the RNA chain to terminate before gene expression. [5] Transcription attenuation is caused by the incorrect formation of a nascent RNA chain.
The term "attenuation" was introduced to describe the his operon. [18] While it is typically used to describe biosynthesis operons of amino acids and other metabolites, programmed transcription termination that does not occur at the end of a gene was first identified in λ phage. [21]
Regulation of transcription thus controls when transcription occurs and how much RNA is created. Transcription of a gene by RNA polymerase can be regulated by several mechanisms. Specificity factors alter the specificity of RNA polymerase for a given promoter or set of promoters, making it more or less likely to bind to them (i.e., sigma ...
Genome damage occurs with a high frequency, estimated to range between tens and hundreds of thousands of DNA damages arising in each cell every day. [53] The process of transcription is a major source of DNA damage, due to the formation of single-strand DNA intermediates that are vulnerable to damage. [53]
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Without the presence of the loop, intrinsic termination is still able to occur. [5] This indicates that the loop is not inherently necessary for intrinsic termination. [citation needed] Generally, the absence of the uracil-rich sequence following the stem-loop will result in a delay or pause in transcription, but termination will not cease ...