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Electron tomography is an extension of traditional transmission electron microscopy and uses a transmission electron microscope to collect the data. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample. This information is collected and used to assemble a ...
The E-T secondary electron detector can be used in the SEM's back-scattered electron mode by either turning off the Faraday cage or by applying a negative voltage to the Faraday cage. However, better back-scattered electron images come from dedicated BSE detectors rather than from using the E–T detector as a BSE detector.
A sample is imaged in a TEM as it is tilted to different angles, resulting in a "tilt-series" of 2D images (top). This tilt-series is then computationally reconstructed into a 3D "tomogram" (bottom). Cryogenic electron tomography ( cryoET ) is an imaging technique used to reconstruct high-resolution (~1–4 nm) three-dimensional volumes of ...
Scanning electron microscope image of a thin TEM sample milled by FIB. The thin membrane shown here is suitable for TEM examination; however, at ~300-nm thickness, it would not be suitable for high-resolution TEM without further milling. More recently focused ion beam methods have been used to prepare samples. FIB is a relatively new technique ...
Cryogenic electron tomography (cryo-ET), a specialized application where many images are taken of individual samples at various tilt angles, resulting in a 3D reconstruction of a single sample. [27] Electron crystallography, method to determine the arrangement of atoms in solids using a TEM
Electron beam computed tomography (EBCT) is a fifth generation computed tomography (CT) scanner in which the X-ray tube is not mechanically spun in order to rotate the source of X-ray photons. This different design was explicitly developed to better image heart structures that never stop moving, performing a complete cycle of movement with each ...
A device used in tomography is called a tomograph, while the image produced is a tomogram. In many cases, the production of these images is based on the mathematical procedure tomographic reconstruction , such as X-ray computed tomography technically being produced from multiple projectional radiographs .
Transmission electron microscopy (TEM) produces high magnification, high resolution images by passing a beam of electrons through a very thin sample. Whereas atomic resolution has been demonstrated with conventional TEM, further improvement in spatial resolution requires correcting the spherical and chromatic aberrations of the microscope lenses .