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Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA ) or plasmid DNA into in vitro cell cultures by lipofection . [ 1 ]
The word liposome derives from two Greek words: lipo ("fat") and soma ("body"); it is so named because its composition is primarily of phospholipid.. Liposomes were first described by British hematologist Alec Douglas Bangham [10] [11] [12] in 1961 at the Babraham Institute, in Cambridge—findings that were published 1964.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
Mediating RNA interference in cultured mammalian cells. Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway.
In the United States each year, roughly 1 in 1,900 people are born with a limb difference, [1] and an additional 185,000 individuals experience a limb amputation. [2] Children's literature with representation of characters with upper limb differences help empower children to accept their own, as well as others', differences. [3]
Children with Down syndrome sometimes have heart problems, frequent ear infections, hypotonia, or undeveloped muscle mass. Children can also be diagnosed with a learning disability, which are disabilities in any of the areas related to language, reading, and mathematics, with basic reading skills being the most common learning disability. The ...
All human societies organize, recognize and classify types of social relationships based on relations between parents, children and other descendants (consanguinity), and relations through marriage . There is also a third type applied to godparents or adoptive children . These culturally defined relationships are referred to as kinship.
Barenaked Ladies began as the duo of Ed Robertson and Steven Page. [3] The two went to school together since Robertson was in grade four (Page was a grade ahead) [6] at Churchill Heights Public School but were not friends until they met each other at a Harvey's restaurant after a Peter Gabriel concert. [6]