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Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. [1] It is used to increase the transfection efficiency of RNA (including mRNA and siRNA ) or plasmid DNA into in vitro cell cultures by lipofection . [ 1 ]
The word liposome derives from two Greek words: lipo ("fat") and soma ("body"); it is so named because its composition is primarily of phospholipid.. Liposomes were first described by British hematologist Alec Douglas Bangham [10] [11] [12] in 1961 at the Babraham Institute, in Cambridge—findings that were published 1964.
Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. [1] [2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.
were missing from each plant in the year 2000 [3]. These chlor-alkali plants have an average of fifty-six cells, each containing as much as 8,000 pounds of mercury [4] and, every year the chlor-alkali industry reports unaccounted for mercury losses to the EPA [5]. Mercury is a danger to unborn children whose developing brains can be damaged if
Mediating RNA interference in cultured mammalian cells. Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded non-coding RNA molecules, typically 20–24 base pairs in length, similar to microRNA (miRNA), and operating within the RNA interference (RNAi) pathway.
In the United States each year, roughly 1 in 1,900 people are born with a limb difference, [1] and an additional 185,000 individuals experience a limb amputation. [2] Children's literature with representation of characters with upper limb differences help empower children to accept their own, as well as others', differences. [3]
Waltham (/ ˈ w ɔː l θ æ m / WAWL-tham) is a city in Middlesex County, Massachusetts, United States, and was an early center for the labor movement as well as a major contributor to the American Industrial Revolution.
The .ibooks format is created with the free iBooks Author e-book layout software from Apple Inc. This proprietary format is based on the EPUB standard, with some differences in the CSS tags used in an ibooks format file, this making it incompatible with the EPUB specification. The End-User Licensing Agreement (EULA) included with iBooks Author ...