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Amylase may be measured in other body fluids, including urine and peritoneal fluid. A January 2007 study from Washington University in St. Louis suggests that saliva tests of the enzyme could be used to indicate sleep deficits , as the enzyme increases its activity in correlation with the length of time a subject has been deprived of sleep.
Specific activity is a measure of enzyme processivity ... The "optimum" temperature for human enzymes is usually between 35 and 40 °C. The average temperature for ...
The test for amylase is easier to perform than that for lipase, making it the primary test used to detect and monitor pancreatitis. Medical laboratories will usually measure either pancreatic amylase or total amylase. If only pancreatic amylase is measured, an increase will not be noted with mumps or other salivary gland trauma.
The enzyme unit, or international unit for enzyme (symbol U, sometimes also IU) is a unit of enzyme's catalytic activity. [1]1 U (μmol/min) is defined as the amount of the enzyme that catalyzes the conversion of one micro mole of substrate per minute under the specified conditions of the assay method.
Because of the closeness in temperatures of peak activity of an α-amylase (63-70 °C) and β-amylase (55-65 °C), the two rests are often performed at once with the time and temperature of the rest determining the ratio of fermentable to non-fermentable sugars in the wort and, hence, the final sweetness of the fermented drink.
The effects of temperature on enzyme activity. Top - increasing temperature increases the rate of reaction (Q 10 coefficient). Middle - the fraction of folded and functional enzyme decreases above its denaturation temperature. Bottom - consequently, an enzyme's optimal rate of reaction is at an intermediate temperature.
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The 0.1 mL of this infusion is equivalent to 0.005 g of malt. Therefore, 5.8 IU/0.005 g of malt = 1160 IU/gram of malt. 100 °L is equivalent to 1160 IU per gram of malt (or 526,176 IU per pound). This value is useful if alternatives to Fehling's reaction are being used to determine the amylase activity.