When.com Web Search

Search results

1. Results From The WOW.Com Content Network

2. RNA-Seq - Wikipedia

   en.wikipedia.org/wiki/RNA-Seq

   hide. Summary of RNA-Seq. Within the organism, genes are transcribed and (in an eukaryotic organism) spliced to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods.

3. Single-cell sequencing - Wikipedia

   en.wikipedia.org/wiki/Single-cell_sequencing

   Single-cell RNA sequencing workflow. Current scRNA-seq protocols involve isolating single cells and their RNA, and then following the same steps as bulk RNA-seq: reverse transcription (RT), amplification, library generation and sequencing. Early methods separated individual cells into separate wells; more recent methods encapsulate individual ...

4. Transcriptome - Wikipedia

   en.wikipedia.org/wiki/Transcriptome

   The three main steps of sequencing transcriptomes of any biological samples include RNA purification, the synthesis of an RNA or cDNA library and sequencing the library. [16] The RNA purification process is different for short and long RNAs. [16] This step is usually followed by an assessment of RNA quality, with the purpose of avoiding ...

5. List of RNA-Seq bioinformatics tools - Wikipedia

   en.wikipedia.org/wiki/List_of_RNA-Seq...

   RNA-Seq [1] [2] [3] is a technique [4] that allows transcriptome studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics tools developed to support the different steps of the process. Here are listed some of the principal tools commonly employed and ...

6. RNA splicing - Wikipedia

   en.wikipedia.org/wiki/RNA_splicing

   Diagram illustrating the two-step biochemistry of splicing. Spliceosomal splicing and self-splicing involve a two-step biochemical process. Both steps involve transesterification reactions that occur between RNA nucleotides. tRNA splicing, however, is an exception and does not occur by transesterification.

7. Reverse transcription polymerase chain reaction - Wikipedia

   en.wikipedia.org/wiki/Reverse_transcription...

   Two-step RT-PCR, as the name implies, occurs in two steps. First the reverse transcription and then the PCR. This method is more sensitive than the one-step method. Kits are also useful for two-step RT-PCR. Just as for one-step PCR, use only intact, high-quality RNA for the best results. The primer for two-step PCR does not have to be sequence ...