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A tiger top tube after centrifugation to separate blood cells from serum. Vacutainer tubes may contain additional substances that preserve blood for processing in a medical laboratory. Using the wrong tube may make the blood sample unusable for the intended purpose. These additives are typically thin film coatings applied using an ultrasonic ...
Tube cap color or type in order of draw Additive Usage and comments Blood culture bottle: Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms: Usually drawn first for minimal risk of contamination. [1] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms ...
The gel in SST II tubes (which appears slightly less opaque) is supposed [weasel words] to have less effect on drug levels in serum. [citation needed] Blood samples should be allowed time to form a clot at room temperature for 30–60 min. [6]
The test tubes in which blood is collected may contain one or more of several additives. In general, tests requiring whole blood call for blood samples collected in test tubes containing some form of the anticoagulant EDTA. EDTA chelates calcium to prevent clotting. EDTA is preferred for hematology tests because it does minimum damage to cell ...
These tubes are commonly sealed with a rubber stopper and often have a specific additive placed in the tube with the stopper color indicating the additive. For example, a blue-top tube is a 5 ml test tube containing sodium citrate as an anticoagulant, used to collect blood for coagulation and glucose-6-phosphate dehydrogenase testing. [5]
a clear solution of blood plasma in the upper phase (which can be separated into its own fractions, see Blood plasma fractionation), the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets in the middle, and; erythrocytes (red blood cells) at the bottom of the centrifuge tube.