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Copper-free click chemistry is a bioorthogonal reaction first developed by Carolyn Bertozzi as an activated variant of an azide alkyne Huisgen cycloaddition, based on the work by Karl Barry Sharpless et al. Unlike CuAAC, Cu-free click chemistry has been modified to be bioorthogonal by eliminating a cytotoxic copper catalyst, allowing reaction ...
Orthogonal protection is a strategy allowing the specific deprotection of one protective group in a multiply-protected structure. For example, the amino acid tyrosine could be protected as a benzyl ester on the carboxyl group, a fluorenylmethylenoxy carbamate on the amine group, and a tert -butyl ether on the phenol group.
The line segments AB and CD are orthogonal to each other. In mathematics, orthogonality is the generalization of the geometric notion of perpendicularity.Whereas perpendicular is typically followed by to when relating two lines to one another (e.g., "line A is perpendicular to line B"), [1] orthogonal is commonly used without to (e.g., "orthogonal lines A and B").
Example of orthogonal factorial design Orthogonality concerns the forms of comparison (contrasts) that can be legitimately and efficiently carried out. Contrasts can be represented by vectors and sets of orthogonal contrasts are uncorrelated and independently distributed if the data are normal.
Another requirement to effectively use 2D liquid chromatography, according to Bushey and Jorgenson, is to employ highly orthogonal techniques which means that the two separation techniques must be as different as possible. [17] Diagram of 1-dimensional LC. An example of the spectral results of this technique is also shown. [15]
The axial pair lie along a common bond axis so that are related by a bond angle of 180°. The equatorial pair of ligands is situated in a plane orthogonal to the axis of the axial pair. Typically the bond distance to the axial ligands is longer than to the equatorial ligands.
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure ().The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence.
Common examples of correlative microscopy techniques are Raman-AFM, [16] [13] Raman-SNOM, [17] and Raman-SEM. [ 18 ] Correlative SEM-Raman imaging is the integration of a confocal Raman microscope into an SEM chamber which allows correlative imaging of several techniques, such as SE, BSE, EDX , EBSD , EBIC , CL , AFM . [ 19 ]