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The main advantage of intravital microscopy is that it allows imaging living cells while they are in the true environment of a complex multicellular organism. Thus, intravital microscopy allows researchers to study the behavior of cells in their natural environment or in vivo rather than in a cell culture. Another advantage of intravital ...
[20] [21] Quantitative phase-contrast microscopy has an advantage over fluorescent and phase-contrast microscopy in that it is both non-invasive and quantitative in its nature. Due to the narrow focal depth of conventional microscopy, live-cell imaging is to a large extent currently limited to observing cells on a single plane.
Multiphoton microscopy (MPM) and second-harmonic generation (SHG) can be used to obtain high-resolution, noninvasive images of thick biological tissues. Researchers are working on the development of small, portable multiphoton systems using femtosecond fiber lasers as a light source, for use in clinical applications and in vivo imaging. [9] [10]
The core technologies being developed and used at the Center are magnetic resonance imaging (MRI) and in vivo magnetic resonance spectroscopy (MRS), magnetoencephalography (MEG) and electroencephalography (EEG), optical imaging techniques (microscopy and near-infrared spectroscopy), positron emission tomography (PET), molecular imaging, medical ...
Molecular imaging with antibodies may be applied to CLE as a diagnostic benchmark due to high correlation with ex vivo microscopy. [21] The molecular imaging technique can be used in a similar manner in the examination of head and neck cancer using CLE, though the diagnostic targets may be different from those in the gastrointestinal tract. [20 ...
In systems biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated cell tracking and feature extraction, drawing many techniques from high-content screening. It is used to study signalling dynamics and behaviour in populations of ...
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