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  2. Confocal microscopy - Wikipedia

    en.wikipedia.org/wiki/Confocal_microscopy

    Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]

  3. Confocal endoscopy - Wikipedia

    en.wikipedia.org/wiki/Confocal_endoscopy

    CLE is a modern, in vivo adaptation of confocal microscopy, the microscopic technique invented by Marvin Minsky in 1957. [10] Since 2004, CLE has been used for observing histopathological changes in gastrointestinal tissues. [10]

  4. Live-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live-cell_imaging

    [20] [21] Quantitative phase-contrast microscopy has an advantage over fluorescent and phase-contrast microscopy in that it is both non-invasive and quantitative in its nature. Due to the narrow focal depth of conventional microscopy, live-cell imaging is to a large extent currently limited to observing cells on a single plane.

  5. Intravital microscopy - Wikipedia

    en.wikipedia.org/wiki/Intravital_microscopy

    Intravital microscopy can be performed using several light microscopy techniques including widefield fluorescence, confocal, multiphoton, spinning disc microscopy and others. The main consideration for the choice of a particular technique is the penetration depth needed to image the area and the amount of cell-cell interaction details required.

  6. Endomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Endomicroscopy

    Endomicroscopy is a technique for obtaining histology-like images from inside the human body in real-time, [1] [2] [3] a process known as ‘optical biopsy’. [4] [5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use.

  7. Two-photon excitation microscopy - Wikipedia

    en.wikipedia.org/wiki/Two-photon_excitation...

    Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.

  8. Raman microscope - Wikipedia

    en.wikipedia.org/wiki/Raman_microscope

    Raman microscopy, and in particular confocal microscopy, can reach down to sub-micrometer lateral spatial resolution. [7] Because a Raman microscope is a diffraction-limited system, its spatial resolution depends on the wavelength of light and the numerical aperture of the focusing element. In confocal Raman microscopy, the diameter of the ...

  9. Photoacoustic imaging - Wikipedia

    en.wikipedia.org/wiki/Photoacoustic_imaging

    Photoacoustic imaging or optoacoustic imaging is a biomedical imaging modality based on the photoacoustic effect.Non-ionizing laser pulses are delivered into biological tissues and part of the energy will be absorbed and converted into heat, leading to transient thermoelastic expansion and thus wideband (i.e. MHz) ultrasonic emission.