Ads
related to: peptide libraries methods and protocols
Search results
Results From The WOW.Com Content Network
Peptide libraries are a popular tool for experiments in drug design, protein–protein interactions, and other biochemical and pharmaceutical applications. Synthetic peptide libraries are synthesized without utilizing biological systems such as phage or in vitro translation. [1] [2] There are at least five subtypes of synthetic peptide ...
A peptide microarray is a planar slide with peptides spotted onto it or assembled directly on the surface by in-situ synthesis. Whereas peptides spotted can undergo quality controls that include mass spectrometer analysis and concentration normalization before spotting and result from a single synthetic batch, peptides synthesized directly on the surface may suffer from batch-to-batch ...
Biopanning is an affinity selection technique which selects for peptides that bind to a given target. [1] All peptide sequences obtained from biopanning using combinatorial peptide libraries have been stored in a special freely available database named BDB. [2] [3] This technique is often used for the selection of antibodies too.
Han et al. described a method that made it possible to keep the advantages of both the high efficiency of S&P synthesis and that of a homogeneous media in the chemical reactions. [13] In their method polyethyleneglycol (PEG) was used as soluble support in S&P synthesis of peptide libraries. MeO-CH 2-CH 2-O-(CH 2-CH 2-O)n-CH 2-CH 2-OH
In 1990 three groups described methods for preparing peptide libraries by biological methods [15] [16] [17] and one year later Fodor et al. published a remarkable method for synthesis of peptide arrays on small glass slides. [18] A "parallel synthesis" method was developed by Mario Geysen and his colleagues for preparation of peptide arrays. [19]
The display of cDNA libraries via phage display is an attractive alternative to the yeast-2-hybrid method for the discovery of interacting proteins and peptides due to its high throughput capability. [ 34 ] pVI has been used preferentially to pVIII and pIII for the expression of cDNA libraries because one can add the protein of interest to the ...