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A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
One major advantage to mass fingerprinting is that it is significantly faster to carry out than peptide sequencing, yet the results are equally useful. [4] Disadvantages include the need for a single protein for analysis and the requirement that the protein sequence is located, at least with significant homology, in a database.
Protein fingerprinting can refer to any of the several methods for identifying or differentiating proteins: Peptide mass fingerprinting, a method developed in 1993 that uses protein mass spectrometry following SDS-PAGE; Older techniques using two-dimensional chromatography and/or protein electrophoresis
Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Pieter Vos, [ 1 ] AFLP uses restriction enzymes to digest genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction ...
Protein purification is a critical process in molecular biology and biochemistry, aimed at isolating a specific protein from a complex mixture, such as cell lysates or tissue extracts. [9] The goal is to obtain the protein in a pure form that retains its biological activity for further study, including functional assays, structural analysis, or ...
The lane labelled "control" is for quality control purposes and contains the DNA fragment but not treated with DNase I. A DNase footprinting assay [1] is a DNA footprinting technique from molecular biology/biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic ...
DNA profiling (also called DNA fingerprinting and genetic fingerprinting) is the process of determining an individual's deoxyribonucleic acid characteristics. DNA analysis intended to identify a species, rather than an individual, is called DNA barcoding .
The DNA footprinting technique can be modified to assess the binding strength of a protein to a region of DNA. Using varying concentrations of the protein for the footprinting experiment, the appearance of the footprint can be observed as the concentrations increase and the proteins binding affinity can then be estimated. [2]