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  2. Reverse transcription polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcription...

    It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will denote Reverse ...

  3. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...

  4. COVID-19 testing - Wikipedia

    en.wikipedia.org/wiki/COVID-19_testing

    RT-PCR can thereby detect SARS-CoV-2, which contains only RNA. The RT-PCR process generally requires a few hours. [24] These tests are also referred to as molecular or genetic assays. [3] Real-time PCR (qPCR) [25] provides advantages including automation, higher-throughput and more reliable instrumentation. It has become the preferred method.

  5. Do you have the flu? Watch out for these 2025 symptoms ... - AOL

    www.aol.com/finance/flu-watch-2025-symptoms-mild...

    The more accurate flu tests—reverse transcription polymerase chain reaction (RT-PCR), viral culture, and immunofluorescence assays—require a health care provider to swipe the back of your ...

  6. Development of COVID-19 tests - Wikipedia

    en.wikipedia.org/wiki/Development_of_COVID-19_tests

    Public Health England announced a test on the 10th, [13] using a real-time RT-PCR (RdRp gene) assay based on oral swabs. [14] The test detected the presence of any type of coronavirus, including specifically identifying SARS-CoV-2. It was rolled out to twelve laboratories across the United Kingdom on 10 February. [15]

  7. Kary Mullis - Wikipedia

    en.wikipedia.org/wiki/Kary_Mullis

    Kary Banks Mullis (December 28, 1944 – August 7, 2019) was an American biochemist.In recognition of his role in the invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith [2] and was awarded the Japan Prize in the same year.

  8. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  9. What does a faint line on a rapid COVID-19 test mean? - AOL

    www.aol.com/faint-line-covid-19-test-225851470.html

    However, people who've had COVID-19 may continue to test positive on PCR tests for up to 90 days, so it may be difficult to use a PCR test to diagnose a new coronavirus infection.