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Chromatographic response function, often abbreviated to CRF, is a coefficient which measures the quality of the separation in the result of a chromatography.. The CRF concept have been created during the development of separation optimization, to compare the quality of many simulated or real chromatographic separations.
Analytical chromatography – the use of chromatography to determine the existence and possibly also the concentration of analyte(s) in a sample. Bonded phase – a stationary phase that is covalently bonded to the support particles or to the inside wall of the column tubing. Chromatogram – the visual output of the chromatograph. In the case ...
Liquid chromatography (LC) is a method that in some ways is more powerful than GC, but can be coupled to mass spectrometry just as easily. In LC, the concerns involving sample preparation can be minimal. In LC, both the stationary and mobile phase can affect the separation, whereas in GC only the stationary phase should be influential.
The previously described work of Martin and Synge impacted the development of the previously known column chromatography and inspired new forms of chromatography such as countercurrent distribution, [9] paper chromatography, [10] and gas-liquid chromatography which is more commonly known as gas chromatography. The modification of silica gel ...
Ion-exchange chromatography (IEC) or ion chromatography (IC) [32] is an analytical technique for the separation and determination of ionic solutes in aqueous samples from environmental and industrial origins such as metal industry, industrial waste water, in biological systems, pharmaceutical samples, food, etc. Retention is based on the ...
A theoretical plate in many separation processes is a hypothetical zone or stage in which two phases, such as the liquid and vapor phases of a substance, establish an equilibrium with each other. Such equilibrium stages may also be referred to as an equilibrium stage , ideal stage , or a theoretical tray .
Chromatography is a physical method of separation that distributes the components you want to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. Cold ethanol precipitation, developed by Cohn in 1946, manipulates pH, ionic strength, ethanol concentration and temperature to ...
Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.