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  2. Retardation factor - Wikipedia

    en.wikipedia.org/wiki/Retardation_factor

    In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor R F is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [2]

  3. Chromatographic response function - Wikipedia

    en.wikipedia.org/wiki/Chromatographic_response...

    Another function is the multispot response function (MRF) as developed by De Spiegeleer et al.{Analytical Chemistry (1987):59(1),62-64} It is based also of differences product. This function always lies between 0 and 1. When two RF values are equal, it is equal to 0, when all RF values are equal-spread, it is equal to 1.

  4. Paper chromatography - Wikipedia

    en.wikipedia.org/wiki/Paper_chromatography

    Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition. In paper chromatography, substances are distributed between a stationary phase and a mobile phase.

  5. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Chromatography, pronounced / ˌ k r oʊ m ə ˈ t ɒ ɡ r ə f i /, is derived from Greek χρῶμα chrōma, which means "color", and γράφειν gráphein, which means "to write".". The combination of these two terms was directly inherited from the invention of the technique first used to separate biological pigme

  6. Resolution (chromatography) - Wikipedia

    en.wikipedia.org/wiki/Resolution_(chromatography)

    Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds.

  7. SDS-PAGE - Wikipedia

    en.wikipedia.org/wiki/SDS-PAGE

    The relative mobility (called Rf value or Rm value) is defined as the distance migrated by the protein band divided by the distance migrated by the buffer front. The distances are each measured from the beginning of the separation gel. The migration of the buffer front roughly corresponds to the migration of the dye contained in the sample buffer.

  8. If You Make Fewer Than 3 Mistakes, It Means You Were ... - AOL

    www.aol.com/fewer-3-mistakes-means-were...

    Whether you loved chemistry or you kind of hated it, we all had to learn the elements of the periodic table at some point, and some of us might still remember it. But how much do we actually remember?

  9. Retention distance - Wikipedia

    en.wikipedia.org/wiki/Retention_distance

    Retention distance, or R D, is a concept in thin layer chromatography, designed for quantitative measurement of equal-spreading of the spots on the chromatographic plate and one of the Chromatographic response functions. It is calculated from the following formula: