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Although the term retention factor is sometimes used synonymously with retardation factor in regard to planar chromatography the term is not defined in this context. However, in column chromatography, the retention factor or capacity factor (k) is defined as the ratio of time an analyte is retained in the stationary phase to the time it is ...
Another function is the multispot response function (MRF) as developed by De Spiegeleer et al.{Analytical Chemistry (1987):59(1),62-64} It is based also of differences product. This function always lies between 0 and 1. When two RF values are equal, it is equal to 0, when all RF values are equal-spread, it is equal to 1.
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
In contrast to the similar concept called Retention uniformity, R d is sensitive to R f values close to 0 or 1, or close to themselves. If two values are not separated, it is equal to 0. For example, the R f values (0,0.2,0.2,0.3) (two compounds not separated at 0.2 and one at the start ) result in R D equal to 0, but R U equal to 0.3609.
Chromatography, pronounced / ˌ k r oʊ m ə ˈ t ɒ ɡ r ə f i /, is derived from Greek χρῶμα chrōma, which means "color", and γράφειν gráphein, which means "to write".". The combination of these two terms was directly inherited from the invention of the technique first used to separate biological pigme
Rf factor may refer to: Chemistry. Retardation factor, a variable measured in chromatography; Biology ... additional terms may apply.
The relative mobility (called Rf value or Rm value) is defined as the distance migrated by the protein band divided by the distance migrated by the buffer front. The distances are each measured from the beginning of the separation gel. The migration of the buffer front roughly corresponds to the migration of the dye contained in the sample buffer.
Also acid ionization constant or acidity constant. A quantitative measure of the strength of an acid in solution expressed as an equilibrium constant for a chemical dissociation reaction in the context of acid-base reactions. It is often given as its base-10 cologarithm, p K a. acid–base extraction A chemical reaction in which chemical species are separated from other acids and bases. acid ...