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The hyaline layer aids cementum in binding to the dentin. [2] The rationale behind the use of enamel matrix derivative proteins, such as Emdogain, in trying to stimulate reproduction of cementum in periodontal bony defects is based on the presence of enamel matrix-like proteins within the hyaline layer.
The hyaline layer, which has an obscure origin, is a clear layer, unlike the granular layer, with a width of up to 20μm. It can have clinical significance during periodontal regeneration. Circumpulpal dentin forms the majority of the dentin and is generally constant in structure.
The hyaline layer is responsible for the adhesion and proper orientation of the cells of an embryo. Throughout development, certain cells change their binding affinity towards hyalin. Hyalin helps cells differentiate into the animal and the vegetal halves during oogenesis by utilizing zinc and lithium ions.
The unique, tubular microscopic appearance of dentin is a result of the formation of dentin around these extensions. [1] After dentin formation begins, the cells of the IEE secrete an organic matrix against the dentin. This matrix immediately mineralizes and becomes the initial layer of the tooth's enamel.
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Tertiary dentin can be divided into reactionary or reparative dentin. Reactionary dentin is formed by odontoblasts when the injury does not damage the odontoblast layer. Reparative dentin is formed by replacement odontoblasts when the injury is so severe that it damages a part of the primary odontoblast layer.