Search results
Results From The WOW.Com Content Network
In whole blood, red blood cells, white blood cells, and platelets are suspended within the plasma. The goal of plasma purification and processing is to extract specific materials that are present in blood, and use them for restoration and repair. There are several components that make up blood plasma, one of which is the protein albumin ...
During the separation, the cell only needs to be suspended in a buffer solution and enter a centrifuge, the whole processes does not involve any chemical (e.g. staining) and physical (e.g. attachment of antibody, lyses of cell membrane) effect on the cells, so the cell will remain unchanged before and after the separation. Because of this, the ...
Often expressed in cm/s. This value is inversely related to the total cross-sectional area of the blood vessel and also differs per cross-section, because in normal condition the blood flow has laminar characteristics. For this reason, the blood flow velocity is the fastest in the middle of the vessel and slowest at the vessel wall.
The cells are first filled with the liquid chosen to be the stationary phase. Under rotation, the pumping of the mobile phase is started, which enters the cells from the inlet. When entering the flow of mobiles phase forms small droplets according to the Stokes' law, which is called atomization. These droplets fall through the stationary phase ...
Using the example of a separation of eukaryotic organelles from intact cells, the cell must first be lysed and homogenized (ideally by a gentle technique, such as Dounce homogenization; harsher techniques or over homogenization will lead to a lower proportion of intact organelles). Once the crude organelle extract is obtained, it may be ...
The Cohn process, developed by Edwin J. Cohn, is a series of purification steps with the purpose of extracting albumin from blood plasma.The process is based on the differential solubility of albumin and other plasma proteins based on pH, ethanol concentration, temperature, ionic strength, and protein concentration.
In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.
Affinity chromatography can be used in a number of applications, including nucleic acid purification, protein purification [9] from cell free extracts, and purification from blood. By using affinity chromatography, one can separate proteins that bind to a certain fragment from proteins that do not bind that specific fragment. [ 10 ]