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The von Kossa histological stain is used to quantify mineralization in cell culture and histological sections. Method. This is a staining method to illustrates ...
von Kossa: Calcium: Calcinosis cutis Pseudoxanthoma elasticum: Wade: Leprosy: Wright: Blood cells: Transient neonatal pustular melanosis [nb 3] Erythema toxicum neonatorum [nb 4] > Granuloma inguinale: Ziehl–Neelsen stain: Leprosy [nb 1]
These reduce silver solution to metallic silver after being exposed to the stain that contains a reductant, for example hydroquinone or formalin. Silver nitrate forms insoluble silver phosphate with phosphate ions; this method is known as the Von Kossa Stain. When subjected to a reducing agent, usually hydroquinone, it forms black elementary ...
H&E stain. Michaelis–Gutmann bodies (M-G bodies) are concentrically layered basophilic inclusions found in Hansemann cells in the urinary tract. These are 2 to 10 μm in diameter, and are thought to represent remnants of phagosomes mineralized by iron and calcium deposits. [citation needed]
Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. [2] While the first cases of IHC staining used fluorescent dyes (see immunofluorescence ), other non-fluorescent methods using enzymes such as peroxidase (see ...
Van Gieson's stain is a mixture of picric acid and acid fuchsin. It is the simplest method of differential staining of collagen and other connective tissue . It was introduced to histology by American neuropsychiatrist and pathologist Ira Van Gieson .
Some major drawbacks of the common protocols for the in-gel digestion are the extended time needed and the multiple processing steps, making the method error-prone with respect to contaminations (especially keratin). These disadvantages were largely removed by the development of optimised protocols and specialised reaction tubes. [7]
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.