Search results
Results From The WOW.Com Content Network
Protein–DNA interactions occur when a protein binds a molecule of DNA, often to regulate the biological function of DNA, usually the expression of a gene. Among the proteins that bind to DNA are transcription factors that activate or repress gene expression by binding to DNA motifs and histones that form part of the structure of DNA and bind ...
Following elution, the protein readily binds DNA, indicating the protein's high affinity for DNA. Histone-like proteins were unknown to be present in bacteria until similarities between eukaryotic histones and the HU-protein were noted, particularly because of the abundancy, basicity, and small size of both of the proteins. [8]
This could include bridging proteins, nucleic acids (DNA or RNA), or other molecules. Bimolecular fluorescence complementation (BiFC) is a new technique in observing the interactions of proteins. Combining with other new techniques, this method can be used to screen protein–protein interactions and their modulators, [3] DERB. [4]
On the other hand, a protein may interact briefly and in a reversible manner with other proteins in only certain cellular contexts – cell type, cell cycle stage, external factors, presence of other binding proteins, etc. – as it happens with most of the proteins involved in biochemical cascades. These are called transient interactions.
DnaA is a protein that activates initiation of DNA replication in bacteria. [1] Based on the Replicon Model, a positively active initiator molecule contacts with a particular spot on a circular chromosome called the replicator to start DNA replication. [2] It is a replication initiation factor which promotes the unwinding of DNA at oriC. [1]
A central intermediate step in this process is the interaction of multiple copies of a recombinase protein with single-stranded DNA to form a DNP filament. Recombinases employed in this process are produced by archaea (RadA recombinase), [16] by bacteria (RecA recombinase) [17] and by eukaryotes from yeast to humans (Rad51 and Dmc1 recombinases ...
Single-stranded DNA is produced during all aspects of DNA metabolism: replication, recombination, and repair. As well as stabilizing this single-stranded DNA, SSB proteins bind to and modulate the function of numerous proteins involved in all of these processes. Active E. coli SSB is composed of four identical 19 kDa subunits. Binding of single ...
SMC proteins are conserved from bacteria to humans. [14] [15] Most bacteria have a single SMC protein in individual species that forms a homodimer.[16] [17] Recently SMC proteins have been shown to aid the daughter cells DNA at the origin of replication to guarantee proper segregation.