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Addgene facilitates the exchange of genetic material between laboratories by offering plasmids and their associated cloning data to non-profit and academic laboratories around the world. Addgene provides a free online database of plasmid cloning information and references, including lists of commonly used vector backbones, popular lentiviral ...
pSC101 is a DNA plasmid that is used as a cloning vector in genetic cloning experiments. pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen. Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells.
The pBR322 plasmid is one of the first plasmids widely used as a cloning vector. Plasmids with specially-constructed features are commonly used in laboratory for cloning purposes . These plasmid are generally non-conjugative but may have many more features, notably a " multiple cloning site " where multiple restriction enzyme cleavage sites ...
An example of a plasmid cloning vector which modifies the inserted protein is pFUSE-Fc plasmid. In order to genetically engineer insulin, the first step is to cut the MCS in the plasmid being used. [8] Once the MCS is cut, the gene for human insulin can be added making the plasmid genetically modified.
The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium. The vector contains features that allow for the convenient insertion of a DNA fragment into the vector or its removal from the vector, for example through the presence of restriction sites.
P1 vectors also contain a P1 plasmid replicon, which ensures only one copy of the vector is present in a cell. However, there is a second P1 replicon- called the P1 lytic replicon- that is controlled by an inducible promoter. This promoter allows the amplification of more than one copy of the vector per cell prior to DNA extraction. [2] bac vector
A schematic representation of the pBR322 vector with restriction sites indicated in blue. pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors . Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco , it was named after Francisco Bolivar Zapata , the postdoctoral researcher ...
The vir helper plasmid contains the vir genes that originated from the Ti plasmid of Agrobacterium. These genes code for a series of proteins that cut the binary vector at the left and right border sequences, and facilitate transfer and integration of T-DNA to the plant's cells and genomes, respectively.