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DTM plate on the right showing color change after two days' growth; Microsporum canis Dermatophyte test medium (DTM) is a specialized agar used in medical mycology. [1] [2] It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator.
A special agar called Dermatophyte Test Medium (DTM) has been formulated to grow and identify dermatophytes. [15] Without having to look at the colony, the hyphae, or macroconidia, one can identify the dermatophyte by a simple color test. The specimen (scraping from skin, nail, or hair) is embedded in the DTM culture medium.
It is a type of dermatophyte that colonizes keratinized tissues (primarily hair) causing infection. [2] The fungus is characterized by its spindle-shaped macroconidia (7–30 × 35–160 μm ), clavate microconidia (2.5–3.5 × 4–7 μm) as well as its pitted or spiny external walls.
Fungal spores may be viewed directly on hair shafts. This technique identifies a fungal infection in about 40%–70% of the infections, but cannot identify the species of dermatophyte. Culture test: This is the most effective, but also the most time-consuming, way to determine if ringworm is on a pet.
Pediococcus acidilactici colonies on an MRS agar plate. De Man–Rogosa–Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study.
KOH test on a vaginal wet mount, showing slings of pseudohyphae of Candida albicans surrounded by round vaginal epithelial cells, conferring a diagnosis of candidal vulvovaginitis. The KOH test , also known as a potassium hydroxide preparation or KOH prep , is a quick, inexpensive fungal test to differentiate dermatophytes and Candida albicans ...
The hair perforation test, also known as an in vitro hair perforation test, is a laboratory test used to help distinguish the isolates of dermatophytes, such as Trichophyton mentagrophytes and its variants. [1] The test is performed by placing an organism into a Petri dish containing water, yeast extract, and hair. After incubation for 4 week ...
Eaton's agar is a type of agar media is used to grow Mycoplasma pneumoniae.. One recipe for the cultivation of M. pneumoniae (Eaton's agar) includes (): [1]. 70% Difco PPLO (pleuropneumonia-like organism) agar or broth base