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  2. 3' mRNA-seq - Wikipedia

    en.wikipedia.org/wiki/3'_mRNA-seq

    3' mRNA-seq methods are generally cheaper per sample than standard bulk RNA-seq methods. [2] [7] [8] [9] This is because of the lower sequencing depth required due to only the 3' end of mRNA molecules being sequenced instead of the whole length of entire transcripts. Read depths of between one million and five million reads are recommended in ...

  3. Transcriptomics technologies - Wikipedia

    en.wikipedia.org/wiki/Transcriptomics_technologies

    The earliest RNA-Seq work was published in 2006 with one hundred thousand transcripts sequenced using 454 technology. [40] This was sufficient coverage to quantify relative transcript abundance. RNA-Seq began to increase in popularity after 2008 when new Solexa/Illumina technologies allowed one billion transcript sequences to be recorded.

  4. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    RNA-Seq can also be used to determine exon/intron boundaries and verify or amend previously annotated 5' and 3' gene boundaries. Recent advances in RNA-Seq include single cell sequencing, bulk RNA sequencing, [6] 3' mRNA-sequencing, in situ sequencing of fixed tissue, and native RNA molecule sequencing with single-molecule real-time sequencing. [7]

  5. Single-cell transcriptomics - Wikipedia

    en.wikipedia.org/wiki/Single-cell_transcriptomics

    RNA Seq Experiment. The single-cell RNA-seq technique converts a population of RNAs to a library of cDNA fragments. These fragments are sequenced by high-throughput next generation sequencing techniques and the reads are mapped back to the reference genome, providing a count of the number of reads associated with each gene. [13]

  6. MicroRNA sequencing - Wikipedia

    en.wikipedia.org/wiki/MicroRNA_sequencing

    MicroRNA sequencing (miRNA-seq), a type of RNA-Seq, is the use of next-generation sequencing or massively parallel high-throughput DNA sequencing to sequence microRNAs, also called miRNAs. miRNA-seq differs from other forms of RNA-seq in that input material is often enriched for small RNAs. miRNA-seq allows researchers to examine tissue-specific expression patterns, disease associations, and ...

  7. Sequence analysis - Wikipedia

    en.wikipedia.org/wiki/Sequence_analysis

    Metagenomics is the study of microbial communities directly obtained from the environment. Different from cultured microorganisms from the lab, the wild sample usually contains dozens, sometimes even thousands of types of microorganisms from their original habitats. [36] Recovering the original genomes can prove to be very challenging.

  8. List of RNA-Seq bioinformatics tools - Wikipedia

    en.wikipedia.org/wiki/List_of_RNA-Seq...

    RNA-Seq [1] [2] [3] is a technique [4] ... Subread can also be used to map DNA-seq reads. ZOOM (commercial) ... analyzing the order of splicing per sample, looking ...

  9. Transcriptome - Wikipedia

    en.wikipedia.org/wiki/Transcriptome

    RNA integrity can also be analyzed quantitatively comparing the ratio and intensity of 28S RNA to 18S RNA reported in the RNA Integrity Number (RIN) score. [23] Since mRNA is the species of interest and it represents only 3% of its total content, the RNA sample should be treated to remove rRNA and tRNA and tissue-specific RNA transcripts.