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The test uses the principles of gel electrophoresis to separate out the various types of hemoglobin and is a type of native gel electrophoresis.After the sample has been treated to release the hemoglobin from the red cells, it is introduced into a porous gel (usually made of agarose or cellulose acetate) and subjected to an electrical field, most commonly in an alkaline medium.
As per guidelines of National AIDS Control Organisation (NACO) for accurate results & mass screening, [citation needed] analysis using hemoglobinometer is a recommended method used for absorbance measurement of whole blood at Hb/HbO2/Isobestic point, [citation needed] based on microcuvette technology such as HemoCue 301 [6] and Mokshit-Chanda ...
He made improvements to the sphygmomanometer, and introduced "Sahli's hemoglobinometer", an instrument used for colorimetric determination of the blood's hemoglobin content. [2] His name is also associated with the "Sahli pipette method" for performing red blood cell counts, as well as the "Hayem-Sahli hemocytometer ", which is a device used to ...
The thiobarbituric acid method (a chemical method specific for the detection of glycation) shows that patients with kidney failure have values for glycated hemoglobin similar to those observed in normal subjects, suggesting that the high values in these patients are a result of binding of something other than glucose to hemoglobin. [50]
All values in Hematology – red blood cells (except hemoglobin in plasma) All values in Hematology – white blood cells; Platelet count (Plt) A few values are for inside red blood cells only: Vitamin B 9 (folic acid/folate) in red blood cells; Mean corpuscular hemoglobin concentration (MCHC)
In the relevant reaction, hydrogen peroxide reacts with the hemoglobin in the blood. Phenolphthalein does not directly participate in this process; instead, it acts as an external source of electrons. In its reaction with hydrogen peroxide, the heme center of hemoglobin behaves as a peroxidase, reducing the peroxide to water. This activity ...