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  2. Bottom–up and top–down design - Wikipedia

    en.wikipedia.org/wiki/Bottom–up_and_topdown...

    Bottom–up and topdown are both strategies of information processing and ordering knowledge, used in a variety of fields including software, humanistic and scientific theories (see systemics), and management and organization. In practice they can be seen as a style of thinking, teaching, or leadership.

  3. Shotgun proteomics - Wikipedia

    en.wikipedia.org/wiki/Shotgun_proteomics

    One example of this is a study by Washburn, Wolters, and Yates in which they used shotgun proteomics on the proteome of a Saccharomyces cerevisiae strain grown to mid-log phase. They were able to detect and identify 1,484 proteins as well as identify proteins rarely seen in proteome analysis, including low-abundance proteins like transcription ...

  4. Top-down proteomics - Wikipedia

    en.wikipedia.org/wiki/Top-down_proteomics

    Top-down vs bottom-up proteomics. Top-down proteomics is a method of protein identification that either uses an ion trapping mass spectrometer to store an isolated protein ion for mass measurement and tandem mass spectrometry (MS/MS) analysis [1] [2] or other protein purification methods such as two-dimensional gel electrophoresis in conjunction with MS/MS. [3] Top-down proteomics is capable ...

  5. Research design - Wikipedia

    en.wikipedia.org/wiki/Research_design

    Research design refers to the overall strategy utilized to answer research questions. A research design typically outlines the theories and models underlying a project; the research question(s) of a project; a strategy for gathering data and information; and a strategy for producing answers from the data. [ 1 ]

  6. Bottom-up - Wikipedia

    en.wikipedia.org/wiki/Bottom-up

    Bottom-up integration testing, in software testing; Top-down and bottom-up design, strategies of information processing and knowledge ordering; Bottom-up proteomics, a laboratory technique involving proteins; Bottom Up Records, a record label founded by Shyheim; Bottom-up approach of the Holocaust, a viewpoint on the causes of the Holocaust

  7. Bottom-up proteomics - Wikipedia

    en.wikipedia.org/wiki/Bottom-up_proteomics

    There is limited protein sequence coverage by identified peptides, loss of labile PTMs, and ambiguity of the origin for redundant peptide sequences. [7] Recently the combination of bottom-up and top-down proteomics, so called middle-down proteomics, is receiving a lot of attention as this approach not only can be applied to the analysis of large protein fragments but also avoids redundant ...

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  9. Top-down - Wikipedia

    en.wikipedia.org/wiki/Top-down

    Top-down and bottom-up design of information ordering; Top-down parsing, a parsing strategy beginning at the highest level of the parse tree Top-down parsing language, an analytic formal grammar to study top-down parsers; Top-down perspective, a camera angle in computer and video games; Top-down shooter, a subgenre of video games