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  2. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    Phenol: The phenol used for biochemistry comes as a water-saturated solution with Tris buffer, as a Tris-buffered 50% phenol, 50% chloroform solution, or as a Tris-buffered 50% phenol, 48% chloroform, 2% isoamyl alcohol solution (sometimes called "25:24:1"). Phenol is naturally somewhat water-soluble, and gives a fuzzy interface, which is ...

  3. Phenol - Wikipedia

    en.wikipedia.org/wiki/Phenol

    Phenol (also known as carbolic acid, phenolic acid, or benzenol) is an aromatic organic compound with the molecular formula C 6 H 5 OH. [5] It is a white crystalline solid that is volatile . The molecule consists of a phenyl group ( −C 6 H 5 ) bonded to a hydroxy group ( −OH ).

  4. Phenol extraction - Wikipedia

    en.wikipedia.org/wiki/Phenol_extraction

    Phenol (C 6 H 5 OH) is a water-soluble compound consisting of a phenyl group (-C 6 H 5) bonded to a hydroxyl group (-OH). Phenol extraction is a widely used technique for purifying nucleic acid samples from cell lysates. [1] To obtain nucleic acids, the cell must be lysed, and the nucleic acids separated from other cell components.

  5. Ferric chloride test - Wikipedia

    en.wikipedia.org/wiki/Ferric_chloride_test

    The sample is dissolved in water, or a mixture of water and ethanol, and a few drops of neutral ferric chloride (FeCl 3) solution, which is prepared by adding de-ionised water. Add sodium hydroxide to the mixture until a permanent brown precipitate is formed.

  6. Naturally occurring phenols - Wikipedia

    en.wikipedia.org/wiki/Naturally_occurring_phenols

    [1] [5] [6] [7]: 104 Some phenols are germicidal and are used in formulating disinfectants. Phenol – the simplest of the phenols Chemical structure of salicylic acid, the active metabolite of aspirin Chemical structure of aloe emodin, a diphenol Quercetin, a typical flavonoid, is a polyphenol Tannic acid, a typical polyphenol of indeterminate ...

  7. Colorimetric analysis - Wikipedia

    en.wikipedia.org/wiki/Colorimetric_analysis

    Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent. It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.

  8. Phenols - Wikipedia

    en.wikipedia.org/wiki/Phenols

    The best-selling drug in the U.S., Acetaminophen, also known as Paracetamol, is a phenol. There are various classification schemes. [15]: 2 A commonly used scheme is based on the number of carbons and was devised by Jeffrey Harborne and Simmonds in 1964 and published in 1980: [15]: 2 [16]

  9. Folin–Ciocalteu reagent - Wikipedia

    en.wikipedia.org/wiki/Folin–Ciocalteu_reagent

    This reagent is distinct from Folin's reagent, which is used to detect amines and sulfur-containing compounds. A 1951 paper entitled "Protein measurement with the Folin phenol reagent" [ 5 ] was the most cited paper in the 1945–1988 Science Citation Index , with 187,652 citations.