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API 20NE rapid test system, one day after sample application. An analytical profile index is a fast identification system based on biochemical incubation tests. Usually, this test is used to quickly diagnose clinically relevant bacteria by allowing physicians to run about 20 tests at one time. [19]
API 20NE Detection system after 24 hours incubation. The analytical profile index, or API, is a classification system for bacteria based on biochemical tests. The system was developed to accelerate the speed of identifying clinically relevant bacteria. It can only be used to identify known species from an index. [1]
The ionised particles are then accelerated, and spectral peaks recorded, producing an expression profile, which is capable of differentiating specific bacterial strains after being compared to known profiles. [26] This includes, in the context of antibiotic susceptibility testing, strains such as beta-lactamase producing E. coli. [9]
The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination.
However, it has retained some clinical usefulness even after the taxonomic changes, [1] and as of 2018, Lancefield designations are still often used to communicate medical microbiological test results. The classification assigns a letter code to each serotype. There are 20 described serotypes assigned the letters A to V (excluding E, I and J). [3]
Blood is normally sterile. [1] The presence of bacteria in the blood is termed bacteremia, and the presence of fungi is called fungemia. [2] Minor damage to the skin [3] or mucous membranes, which can occur in situations like toothbrushing or defecation, [4] [5] can introduce bacteria into the bloodstream, but this bacteremia is normally transient and is rarely detected in cultures because the ...
It was developed in 1953 by Hugh and Leifson to be utilized in microbiology to determine the way a microorganism metabolizes a carbohydrate such as glucose (dextrose). [1] OF-glucose deeps contain glucose as a carbohydrate, peptones, bromothymol blue indicator for Hugh-Leifson's OF medium or phenol red for King's OF medium, and 0.5% agar.
Voges–Proskauer / ˈ f oʊ ɡ ə s ˈ p r ɒ s k aʊ. ər / or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which is a glucose-phosphate broth that has been inoculated with bacteria. A cherry red color indicates a positive ...