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The ionised particles are then accelerated, and spectral peaks recorded, producing an expression profile, which is capable of differentiating specific bacterial strains after being compared to known profiles. [26] This includes, in the context of antibiotic susceptibility testing, strains such as beta-lactamase producing E. coli. [9]
As other virulent bacteria, GBS harbors an important number of virulence factors (virulence factors are molecules produced by bacteria that boosts their capacity to infect and damage human tissues), the most important being the capsular polysaccharide (rich in sialic acid) [3] [19] and a pore-forming toxin, β-hemolysin.
Before starting a test, one must confirm the cultured bacteria are Enterobacteriaceae, this is done by a quick oxidase test for cytochrome coxidase. Enterobacteriaceae are typically oxidase negative, meaning they either do not use oxygen as an electron acceptor in the electron transport chain , or they use a different cytochrome enzyme for ...
The CSF/serum glucose ratio, also known as CSF/blood glucose ratio, is a measurement used to compare CSF glucose and blood sugar. Because many bacteria metabolize glucose, and because the blood–brain barrier minimizes transversal, the ratio can be useful in determining whether there is a bacterial infection in the CSF. The normal ratio is 0.6 ...
Streak a beta-lysin–producing strain of aureus down the center of a sheep blood agar plate. The test organism streak should be 3 to 4 cm long. Streak test organisms across the plate perpendicular to the S. aureus streak within 2 mm. (Multiple organisms can be tested on a single plate). Incubate at 35°-37°C in ambient air for 18-24 hours.
McFarland standards. No. 0.5, 1 and 2. In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize microbial testing.
The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which is a glucose-phosphate broth that has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result. [1] The test depends on the digestion of glucose to ...
Granada medium is a selective and differential culture medium designed to selectively isolate Streptococcus agalactiae (Group B streptococcus, GBS) and differentiate it from other microorganisms.