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Isoelectric point. The isoelectric point (pI, pH (I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. The standard nomenclature to represent the isoelectric point is pH (I). [1] However, pI is also used. [2] For brevity, this article uses pI.
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). [1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the pH ...
Protein precipitation. Protein precipitation is widely used in downstream processing of biological products in order to concentrate proteins and purify them from various contaminants. For example, in the biotechnology industry protein precipitation is used to eliminate contaminants commonly contained in blood. [1]
The two dimensions that proteins are separated into using this technique can be isoelectric point, protein complex mass in the native state, or protein mass. [citation needed] The separation by isoelectric point is called isoelectric focusing. Thereby, a pH gradient is applied to a gel and an electric potential is applied across the gel, making ...
The point of zero charge is the pH value for which the net surface charge of adsorbent is equal to zero. This concept has been introduced by an increase of interest in the pH of the solution during adsorption experiments. [1] The reason is that the adsorption of some substances is very dependent on pH. The pzc value is determined by the ...
The pH at which the average charge is zero is known as the molecule's isoelectric point. Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing. Metal oxides which react with both acids as well as bases to produce salts and water are known as amphoteric oxides.
Ion chromatography. Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [2] large proteins, [3] small nucleotides, [4] and amino acids.
Isoelectric. Isoelectric may refer to: Isoelectric point, the pH at which a particular molecule carries no net electrical charge. Isoelectric focusing, a technique for separating different molecules by differences in their isoelectric point. Isoelectric line representing the absence of electrical activity on an electrocardiogram.