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Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Camelids are unique among mammals as they also have fully functional antibodies which have two heavy chains, but lack the light chains usually paired with each heavy chain. [ 5 ] Sharks also possess, as part of their adaptive immune systems, a functional heavy-chain homodimeric antibody-like molecule referred to as IgNAR (immunoglobulin new ...
One of the main difficulties with IHC staining is overcoming specific or non-specific background. Optimisation of fixation methods and times, pre-treatment with blocking agents, incubating antibodies with high salt, and optimising post-antibody wash buffers and wash times are all important for obtaining high quality immunostaining.
The immunohistochemistry (IHC) test is a laboratory method that detects antibodies of prions (mis-shapen proteins thought to transmit bovine spongiform encephalopathy, BSE or mad cow disease) by exposing a brain sample to a stain that appears as a specific color under a microscope.
For low and high protein overexpression/gene amplification, CISH and IHC show a concordance of over 86% and over 89%, respectively. [18] It has been shown that monoclonal antibodies are better than polyclonal antibodies for detection in both IHC and CISH as they bind more specifically, which leads to a higher concordance rate. [18]
This is a recombinant monoclonal antibody to Macaque pan-species IgG. This is used in ELISA to detect anti-rhesus mouse antibodies in serum. The antibody is able to distinguish between human and macaque IgG antibodies. In HIV research it has been used to detect macaque antibodies while against IV-administered HIV broadly neutralizing antibodies ...