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Fluorescence shifts energy in the incident illumination from shorter wavelengths to longer (such as blue to yellow) and thus can make the fluorescent color appear brighter (more saturated) than it could possibly be by reflection alone.
A fluorescent lamp, or fluorescent tube, is a low-pressure mercury-vapor gas-discharge lamp that uses fluorescence to produce visible light. An electric current in the gas excites mercury vapor, to produce ultraviolet and make a phosphor coating in the lamp glow.
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
Jablonski diagram shows the energy levels in a fluorescing atom in a phosphor. An electron in the phosphor absorbs a high-energy photon from the applied radiation, exciting it to a higher energy level. After losing some energy in non-radiative transitions, it eventually transitions back to its ground state energy level by fluorescence, emitting ...
This is a list of sources of light, the visible part of the electromagnetic spectrum.Light sources produce photons from another energy source, such as heat, chemical reactions, or conversion of mass or a different frequency of electromagnetic energy, and include light bulbs and stars like the Sun. Reflectors (such as the moon, cat's eyes, and mirrors) do not actually produce the light that ...
Furthermore, tryptophan fluorescence is strongly influenced by the proximity of other residues (i.e., nearby protonated groups such as Asp or Glu can cause quenching of Trp fluorescence). Also, energy transfer between tryptophan and the other fluorescent amino acids is possible, which would affect the analysis, especially in cases where the ...
Chemiluminescence is different from fluorescence. Hence, fluorescent proteins such as Green fluorescent protein are not chemiluminescent. However, combining GFP with luciferases allows bioluminescence resonance energy transfer (BRET), which increases the quantum yield of light emitted in these systems.
This transfer of energy between different scales requires that the dynamics of the system is nonlinear. Strictly speaking, a cascade requires the energy transfer to be local in scale (only between fluctuations of nearly the same size), evoking a cascading waterfall from pool to pool without long-range transfers across the scale domain.