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Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat.
Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...
A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left ...
Subsequently, it is cut frozen with the microtome portion of the cryostat, the section is picked up on a glass slide and stained (usually with hematoxylin and eosin, the H&E stain). The preparation of the sample is much more rapid than with traditional histology technique (around 10 minutes vs 16 hours). However, the technical quality of the ...
The field of histology that includes the preparation of tissues for microscopic examination is known as histotechnology. Job titles for the trained personnel who prepare histological specimens for examination are numerous and include histotechnicians, histotechnologists, [ 11 ] histology technicians and technologists, medical laboratory ...
The general steps of affixing paraffin sections can be concluded as 1. Clean the required slides, 2. Mark the cleaned slides, 3. Drop affixative on each slide, 4. Put on another slide, 5. Spread the affixative, 6. Drop floating medium, 7. Divide the paraffin into required length, 8. Transfer the sections, 9.
Digital pathology is a sub-field of pathology that focuses on managing and analyzing information generated from digitized specimen slides. It utilizes computer-based technology and virtual microscopy to view, manage, share, and analyze digital slides on computer monitors. [ 1 ]
Gross examination of a kidney (right of image) with a renal oncocytoma (left of image).. Gross processing, "grossing" or "gross pathology" is the process by which pathology specimens undergo examination with the bare eye to obtain diagnostic information, as well as cutting and tissue sampling in order to prepare material for subsequent microscopic examination.