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Example of a T-REx system controlling the expression of shRNA. Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline).
The tac promoter consists of the '–35' region of the trp promoter and the '–10' region of the lac promoter (and differs from a related trc promoter by 1 bp [3]). The tac promoter is, therefore, inducible by IPTG (Isopropyl β- D -1-thiogalactopyranoside), whilst also allowing higher maximum gene expression than either the lac or trp promoters.
Soon, the lab was able to clone the T7 RNA polymerase and use it, along with the powerful T7 promoter, to transcribe copious amounts of almost any gene. [4] The development of the T7 expression system has been considered the most successful biotechnology developed at the Brookhaven National Laboratory, being licensed by over 900 companies which ...
Activator-binding sites may be located very close to the promoter or numerous base pairs away. [2] [3] If the regulatory sequence is located far away, the DNA will loop over itself (DNA looping) in order for the bound activator to interact with the transcription machinery at the promoter site. [2] [3]
The Pribnow box has a function similar to the TATA box that occurs in promoters in eukaryotes and archaea: it is recognized and bound by a subunit of RNA polymerase during initiation of transcription. [3] This region of the DNA is also the first place where base pairs separate during prokaryotic transcription to allow access to the template strand.
EPD (Eukaryotic Promoter Database) is a biological database and web resource of eukaryotic RNA polymerase II promoters with experimentally defined transcription start sites. [1] Originally, EPD was a manually curated resource relying on transcript mapping experiments (mostly primer extension and nuclease protection assays ) targeted at ...
The sequence identified overlapped a binding site for GAL4 protein, which is a positive regulator for transcription which coincides with the function of upstream activating sequences. [9] Another study looked at the effect of inserting the UAS G into the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (GPD) . This hybrid ...
The Mammalian Promoter Database (MPromDb) is a curated database of gene promoters identified from ChIP-seq. [1] The proximal promoter region (upstream of the core-promoter region) contains the cis-regulatory elements of most of the transcription factors (TFs).