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Chocolate agar is a type of blood agar plate in which the blood cells have been lysed by heating the cells to 80 °C. It is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae. Chocolate agar is named for its color, and no chocolate is contained in the plate.
Green tea-flavored yōkan, a popular Japanese red bean jelly made from agar A blood agar plate used to culture bacteria and diagnose infection. Agar (/ ˈ eɪ ɡ ɑːr / or / ˈ ɑː ɡ ər /), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from “ogonori” and “tengusa”.
Blood is normally sterile. [1] The presence of bacteria in the blood is termed bacteremia, and the presence of fungi is called fungemia. [2] Minor damage to the skin [3] or mucous membranes, which can occur in situations like toothbrushing or defecation, [4] [5] can introduce bacteria into the bloodstream, but this bacteremia is normally transient and is rarely detected in cultures because the ...
Chocolate agar (CHOC) or chocolate blood agar (CBA) is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. [ 1 ] [ 2 ] [ 3 ] It is a variant of the blood agar plate , containing red blood cells that have been lysed by slowly heating to 80°C.
Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. This is particularly useful in classifying streptococcal species. A substance that causes hemolysis is called a hemolysin.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Haemophilus influenzae satellite colonies (pin points) near Staphylococcus aureus (yellow) on blood agar plate. Bacterial culture of H. influenzae is performed on agar plates. The strongest growth is seen on chocolate agar at 37 °C in a CO 2-enriched incubator. [33] The ideal CO 2 concentration for the culture is ~5%. [34]
Chocolate agar is an excellent Haemophilus growth medium, as it allows for increased accessibility to these factors. [8] Alternatively, Haemophilus is sometimes cultured using the "Staph streak" technique: both Staphylococcus and Haemophilus organisms are cultured together on a single blood agar