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Washed red blood cells are red blood cells that have had most of the plasma, platelets and white blood cells removed and replaced with saline or another type of preservation solution. [ 1 ] [ 2 ] The most common reason for using washed red blood cells in transfusion medicine is to prevent the recurrence of severe allergic transfusion reactions ...
[44] [45] Washing typically consists of diluting the red blood cells in saline-based washing solution or red blood cell additive solutions and then washing away any remaining plasma and debris during one or several centrifugation cycles. The process can be performed manually, or with an automated cell washer or processor.
The washed blood can be labeled in the circular bag or transferred into a more traditionally shaped blood storage bag. Because the washing process creates an open system, red blood cells washed with this machine expire after 24 hours when kept at 1-6 °C. [2]
Regardless of manufacturer, there are many types of cell processors. Cell processors are red cell washing devices that collect anticoagulated shed or recovered blood, wash and separate the red blood cells (RBC) by centrifugation or filtration such as the HemoClear filter. After, the washed RBCs can be returned to the same patient by reinfusion.
To perform the test, a blood sample is taken and the red blood cells are washed (removing the patient's plasma and unbound antibodies from the red blood cells) and then incubated with anti-human globulin ("Coombs reagent"). If the red cells then agglutinate, the test is positive, a visual indication that antibodies or complement proteins are ...
There are two main ways to salvage red blood cells. Cell processing and direct injection. Cell processors are red cell washing devices like the Cell Saver that collect anticoagulated shed or recovered blood, wash and separate the red blood cells (RBC) by centrifugation. Filtration devices like HemoClear microfilter, constitute the second major ...
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Blood may be aspirated from the surgical site, processed and reinfused with the surgeon's consent. If an inadequate supply of blood exists, the washed red cells may be used to support the patient's vital signs with the surgeon's consent. The use of leukocyte reduction filters is recommended. [citation needed]