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The action spectra of chlorophyll molecules are slightly modified in vivo depending on specific pigment-protein interactions. An action spectrum is a graph of the rate of biological effectiveness plotted against wavelength of light. [1] It is related to absorption spectrum in many systems.
Photochemical action plots are a scientific tool used to understand the effects of different wavelengths of light on photochemical reactions. The methodology involves exposing a reaction solution to the same number of photons at varying monochromatic wavelengths, monitoring the conversion or reaction yield of starting materials and/or reaction ...
Growth towards a light source is called positive phototropism, while growth away from light is called negative phototropism. Negative phototropism is not to be confused with skototropism, which is defined as the growth towards darkness, whereas negative phototropism can refer to either the growth away from a light source or towards the darkness ...
Diagram illustrating the light path through a dark-field microscope. The steps are illustrated in the figure where an inverted microscope is used. Light enters the microscope for illumination of the sample. A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide ...
Other larvae show negative phototaxis by swimming away from the light. First, the light comes from left and then from the right side. When the light direction is switched the larvae turn. The side where the light is coming from is indicated by a white bar. The larvae display mixed phototaxis, some negatively phototactic larvae are tracked.
For example, in green plants, the action spectrum resembles the absorption spectrum for chlorophylls and carotenoids with absorption peaks in violet-blue and red light. In red algae, the action spectrum is blue-green light, which allows these algae to use the blue end of the spectrum to grow in the deeper waters that filter out the longer ...
The wavelength of light is then selected by the slit on the upper right corner. An optical spectrometer (spectrophotometer, spectrograph or spectroscope) is an instrument used to measure properties of light over a specific portion of the electromagnetic spectrum, typically used in spectroscopic analysis to identify materials. [1]
The light path of a bright-field microscope is extremely simple; no additional components are required beyond the normal light-microscope setup. The light path begins at the illuminator or the light source on the base of the microscope. Often a halogen lamp is used. The light travels through the objective lens into the ocular lens, through ...