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3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions. The 3T3-L1 Swiss subclone line has been widely utilized, since its ...
3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green .
In vitro studies on differentiation have used the pre-committed preadipocyte lineage, such as 3T3-L1 and 3T3-F442A cell line, or preadipocytes isolated from the stromal-vascular fraction of white adipose tissue. In vitro differentiation is a highly ordered process. Firstly, proliferating preadipocytes arrest growth usually by contact inhibition.
Co-culturing 3T3-L1 preadipocytes in a 3D space with murine endothelial bEND.3 cells can create a vascular-like network assembly with concomitant lipogenesis in perivascular cells (refer to the attached figure). [8] In addition to cell lines, organogenesis of white adipose tissue (WAT) can be simulated from primary cells. [8]
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Lane's laboratory published widely cited early studies of the insulin receptor and made extensive use of the 3T3-L1 cell line for investigating cellular differentiation processes leading to adipocytes. After the discovery of the satiety-regulating hormone leptin, Lane's laboratory focused its efforts on characterizing its regulation. [2] [3]
Chemerin has been implicated in autocrine / paracrine signaling for adipocyte differentiation and also stimulation of lipolysis. [11] [12] Studies with 3T3-L1 cells have shown chemerin expression is low in pre-differentiated adipocytes [11] but its expression and secretion increases both during and after differentiation in vitro.
Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis. [35] One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA.