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Bacteremia is most commonly diagnosed by blood culture, in which a sample of blood drawn from the vein by needle puncture is allowed to incubate with a medium that promotes bacterial growth. [33] If bacteria are present in the bloodstream at the time the sample is obtained, the bacteria will multiply and can thereby be detected.
Blood is normally sterile. [1] The presence of bacteria in the blood is termed bacteremia, and the presence of fungi is called fungemia. [2] Minor damage to the skin [3] or mucous membranes, which can occur in situations like toothbrushing or defecation, [4] [5] can introduce bacteria into the bloodstream, but this bacteremia is normally transient and is rarely detected in cultures because the ...
The aminopeptidase test analyzes bacteria for the production of the enzyme L-alanine-aminopeptidase, an enzyme found in many gram-negative bacteria. Adding L-Alanine-4-nitroanilide hydrochloride to a bacterial culture works as an indicator, changing to a yellow color in the presence of L-alanine-aminopeptidase.
The ionised particles are then accelerated, and spectral peaks recorded, producing an expression profile, which is capable of differentiating specific bacterial strains after being compared to known profiles. [26] This includes, in the context of antibiotic susceptibility testing, strains such as beta-lactamase producing E. coli. [9]
Several tests were performed to determine carbohydrate and physiological reactions, peptidoglycan type, the chemical makeup of the teichoic acids in the cell wall, and cytochrome pattern. [1] All the strains were found to be facultative anaerobes and used glucose to produce equivalent amounts of D- and L-lactate. [ 1 ]
Some bacteria have cell walls which are particularly adept at retaining stains. These will appear positive by Gram stain even though they are not closely related to other gram-positive bacteria. These are called acid-fast bacteria, and can only be differentiated from other gram-positive bacteria by special staining procedures. [25]
When some otherwise non-hemolytic bacteria, such as strains of the cholera-causing bacteria Vibrio cholerae, are plated on blood agar, no clearings are observed surrounding isolated colonies, but the blood surrounding larger areas of growth turns green. This process, called hemodigestion, is caused by the metabolic by-products of the bacteria ...
Streak a beta-lysin–producing strain of aureus down the center of a sheep blood agar plate. The test organism streak should be 3 to 4 cm long. Streak test organisms across the plate perpendicular to the S. aureus streak within 2 mm. (Multiple organisms can be tested on a single plate). Incubate at 35°-37°C in ambient air for 18-24 hours.