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In ultra-high-throughput sequencing as many as 500,000 sequencing-by-synthesis operations may be run in parallel. [91] [92] [93] Such technologies led to the ability to sequence an entire human genome in as little as one day. [94] As of 2019, corporate leaders in the development of high-throughput sequencing products included Illumina, Qiagen ...
Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing.
[4] [16] [17] Fragments of this size are suitable for high-throughput sequencing. [4] [16] [17] Following sonication, fragments can be size selected using AMPure XP beads from Beckman Coulter to obtain ligation products with a size distribution between 150 and 300 bp. [4] [17] This is the optimal fragment size window for HiSeq cluster formation ...
High-throughput screening (HTS) is a method for scientific discovery especially used in drug discovery and relevant to the fields of biology, materials science [1] and chemistry. [ 2 ] [ 3 ] Using robotics , data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to ...
Hi-C uses high-throughput sequencing to find the nucleotide sequence of fragments [2] [22] and uses paired end sequencing, which retrieves a short sequence from each end of each ligated fragment. As such, for a given ligated fragment, the two sequences obtained should represent two different restriction fragments that were ligated together in ...
In its modern inception, high-throughput genome sequencing involves fragmenting the genome into small single-stranded pieces, followed by amplification of the fragments by polymerase chain reaction (PCR). Adopting the Sanger method, each DNA fragment is irreversibly terminated with the incorporation of a fluorescently labeled dideoxy chain ...