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A high vaginal swab (HVS) is a medical procedure performed in obstetrics and gynaecology to test vaginal discharge for the presence of vaginal thrush, bacterial vaginosis and trichomonas vaginalis. [1] [2] [3] It is carried out in clean conditions, by a healthcare professional who uses a speculum to look at the cervix and vagina.
Trichomonas vaginalis from a vaginal swab. This is a heavy infection; there were probably thousands of trichomonads in the vagina. Alfred Francois Donné (1801–1878) was the first to describe a procedure to diagnose trichomoniasis through "the microscopic observation of motile protozoa in vaginal or cervical secretions" in 1836.
The Nugent Score is a Gram stain scoring system for vaginal swabs to diagnose bacterial vaginosis (BV).The Nugent score is calculated by assessing for the presence of large Gram-positive rods (Lactobacillus morphotypes; decrease in Lactobacillus scored as 0 to 4), small Gram-variable rods (Gardnerella vaginalis morphotypes; scored as 0 to 4), and curved Gram-variable rods (Mobiluncus spp ...
Micrograph of a clue cell (center), covered in bacteria, as compared to an unremarkable squamous cell at bottom left, and a neutrophil at bottom center. Pap stain Phase contrast microscopy of clue cells in a vaginal swab. Clue cells are epithelial cells of the vagina that get their distinctive stippled appearance by being covered with bacteria ...
Bacterial vaginosis is usually confirmed by a Gram stain of vaginal secretions. [40] A characteristic "fishy" odor on wet mount. This test, called the whiff test, is performed by adding a small amount of potassium hydroxide to a microscope slide containing the vaginal discharge.
Trichomonas is diagnosed by looking at a sample of discharge under the microscope showing trichomonads moving on the slide. [28] However, in women with trichomonas the organism is typically detected in only 60-80% of cases. [28] Other testing, including a culture of the discharge or a PCR assay, are more likely to detect the organism. [28]