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This file is licensed under the United Kingdom Open Government Licence v3.0.: You are free to: copy, publish, distribute and transmit the Information; adapt the Information; ...
Schematic diagram of the arrangement of optical components in a typical Spectrofluorometer. A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.
Ruby spectra R1, R2 lines. The ruby fluorescence pressure scale is an optical method to measure pressure within a sample chamber of a diamond anvil cell apparatus. [1] Since it is an optical method, which fully make use of the transparency of diamond anvils and only requires an access to a small scale laser generator, it has become the most prevalent pressure gauge method in high pressure ...
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
If the instrument is designed to measure the spectrum on an absolute scale rather than a relative one, then it is typically called a spectrophotometer. The majority of spectrophotometers are used in spectral regions near the visible spectrum. A spectrometer that is calibrated for measurement of the incident optical power is called a ...
At some point in time, the flow is suddenly stopped, and the reaction is monitored using a suitable spectroscopic probe, such as absorbance, fluorescence or fluorescence polarization. The change in spectroscopic signal as a function of time is recorded, and the rate constants that define the reaction kinetics can then be obtained by fitting the ...
In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly ...